WebbIn particular, use of the thermostabilized apocytochrome b562RIL as a fusion partner displays certain advantages over previously utilized fusion proteins, resulting in a significant improvement in stability and structure of GPCR-fusion constructs. © 2012 Elsevier Ltd All rights reserved. Register to see more suggestions WebbThe T4 lysozyme (T4L) and thermostabilized apocytochrome b 562RIL (BRIL) tags were chosen as their molecular weight was only slightly larger than CRIP1a. The T4L protein contained the following site-directed changes to inactivate the enzyme (E11Q and D20N) and to remove Cys residues (C54T and C97A) to prevent thiol crosslinking (13, 14).
Structure of the nociceptin/orphanin FQ receptor in complex with a …
Webb24 feb. 2024 · To overcome these technical difficulties, we fused the channel with a C-terminal BRIL (thermostabilized apocytochrome b 562 RIL), a four-helix bundle protein that has been widely used as a crystallization chaperone to improve membrane protein stability and to promote crystal formation ( 33, 34 ). Webb12 juli 2024 · In Figure S1, we describe the construct xSMO-BRIL-glycKO consisting of Xenopus laevis SMO (xSMO) with its intracellular loop 3 (ICL3) replaced by … dean schiller stream
Solubilization, purification, and ligand binding ... - PeerJ
Webb1 okt. 2003 · —Hydrophobicity profiles of polypeptides used in allotopic expression studies. The Kyte and Doolittle plots illustrate the highly hydrophobic character of the mitochondrial-coded apocytochrome b (A), ND4 (C), and to a lesser extent ATPase 8 (B). For comparison purposes the profiles of the two controls used in the in vivo import … Webb12 aug. 2024 · In this study, we developed a novel method to express the full-length human UGT2B7 using the Bac-to-Bac baculovirus expression system, by introducing the … WebbUpper bands correspond to the crosslinked complex and lower bands to free receptor. Crosslinking of thermostabilized apocytochrome b562 (bRIL)-R197C-ACKR3 with Cys mutants in the N terminus of CXCL12. No crosslinking is seen for control samples N45C-CXCL12 or WT-CXCL12. ( b ) Crosslinking of Y7C-CXCL12 with ECL2 mutants of ACKR3. generate invoices online free