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Webemission filter (Semrock, BLP01-488R-25), and was detected by the EM-CCD camera. The fluorescence images were recorded at 133 Hz (i.e., 7.5 ms exposure time). The pixel size of the images was 160 nm. Image analysis The diffusion rate and mode of 1 and 2a+2b were analysed by single-molecule localization and tracking method.23 First, the WebFluorescence is collected by the same optics, passes through the DM and long pass filter (LPF, BLP01-488R-25, Semrock), is focusedby lens L5(355151-A,Thorlabs)into a 1 meter long multi-mode fiber (MMF, M31L01, Thorlabs) for delivery to a photomultipliertube (PMT) detector (H7422-40,Hamamatsu). trinette wacker
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Webthrough a 488 nm long-pass edge filter (Semrock; BLP01- 488R-25), and characterized using an Ocean Optics QE Pro spectrometer. Figure 1: Schematic of the measurement setup. 2.3 β-hydroxybutyrate (BHB) characterization solutions. A 0.5 mM BHB stock solution was prepared by reconstituting the WebBLP01-488R-25 EdgeBasic filters offer an ideal combination of performance and value for applications including Raman spectroscopy and fluorescence imaging and measurements. WebNewOpto offers Raman Filter, which gives a superb combination of performance and value for application in Raman spectroscopy and fluorescence imaging and measurements. … trinette wilson